Dr. Dao Nguyen, now at McGill University, trained in the University of Washington lab of Dr. Pradeep Singh, a lung specialist who studies bacterial biofilm infections.

“A chief cause of the resistance of biofilms is that bacteria on the outside of the clusters have the first shot at the nutrients that diffuse in,” said Pradeep Singh, associate professor of medicine and microbiology at the University of Washington.

“This produces starvation of the bacteria inside clusters, and severe resistance to (their) killing,” added the senior study author, the journal Science reports.

“Bacteria become starved when they exhaust nutrient supplies in the (infected) body, or if they live clustered together in groups known as biofilms,” said study co-author Dao Nguyen, assistant professor of medicine at Montreal’s McGill University.

Preventing pathogenic bacteria from sensing nutrient starvation may present a new therapeutic approach to increasing antibiotic efficacy and preventing drug resistance, researchers claim. A team led by McGill University investigators has found that blocking an active mechanism used by bacteria to respond to starvation by slowing their growth significantly reduces the natural tolerance to antibiotics that infectious organisms develop when nutrient supplies become low.

The investigators work is reported in Science in a paper titled “Active Starvation Responses Mediate Antibiotic Tolerance in Biofilms and Nutrient-Limited Bacteria.” Pradeep K. Singh, Ph.D., Dao Nguyen, Ph.D., and colleagues

Abstract:

Bacteria become highly tolerant to antibiotics when nutrients are limited. The inactivity of antibiotic targets caused by starvation-induced growth arrest is thought to be a key mechanism producing tolerance. Here we show that the antibiotic tolerance of nutrient-limited and biofilm Pseudomonas aeruginosa is mediated by active responses to starvation, rather than by the passive effects of growth arrest. The protective mechanism is controlled by the starvation-signaling stringent response (SR), and our experiments link SR-mediated tolerance to reduced levels of oxidant stress in bacterial cells. Furthermore, inactivating this protective mechanism sensitized biofilms by several orders of magnitude to four different classes of antibiotics and markedly enhanced the efficacy of antibiotic treatment in experimental infections.

You can read all of my biofilm posts here

The below cycle of inflammation (also known as an inflammatory cascade) is one of the most profound contributors to ill health and disease we face. Once set in motion, this inflammatory cycle will feed itself and becomes harder to suppress. 75% of the patients I see are in my practice because of the effects inflammation is having on their body. 100% of these effects are a direct result of not following (step 6) at the bottom of the page. The easiest way to avoid disease and ill health is to remove as many stressors (step 1) out of your life.Step 1. Over-exposure or excessive physical, emotional and/or chemical stress’, leading to the activation of  a potent modulator of the inflammatory cycle.

Stressors: Radiation; oxidative stress; physical injury, bacterial endotoxin or lipopolysaccharide (LPS) from food-borne bacteria (E. coli and Salmonella); “Leaky Gut Syndrome”; food and environmental allergens; viral infections; excessive consumption of simple sugars (sucrose); consumption of corn oil; arachidonic acid metabolites such as PGE2; nutrient poor, processed food diet; insufficient Omega 3 fatty acids (ALA, EPA, DHA), insufficient GLA and Vitamin D deficiency.

Step 2. Activation of NF-κB

NF-κB is widely used by eukaryotic (cells that contain a nucleus) cells as a regulator of genes that control cell proliferation and cell survival (anti-apoptotic). Blocking NF-κB can cause tumor cells to stop proliferating, to die, or to become more sensitive to the action of anti-tumor agents.

NF-κB also controls many genes involved in inflammation (cytokines [signaling molecules used extensively in intercellular communication], cell adhesion molecules and the pro-inflammatory enzymes: inducible nitric oxide synthase (iNOS,) cyclo-oxygenase (COX) and lipoxygenase (LPOX). NF-κB is found to be chronically active in many inflammatory diseases, such as inflammatory bowel disease, arthritis, sepsis, gastritis, asthma, neurodegenerative diseases, and cardiovascular disease.

Step 3. Cytokines, cell adhesion molecules and the pro-inflammatory enzymes are produced.

*Interleukin-6 (IL-6) → C-Reactive Protein (CRP)
Cyclooxygenase-2 → Prostaglandin-E2 (PGE2) and Thromboxanes
Interleukin-1 (IL-1) → Collagenase-3 (MMP-13)
Lipoxygenase → Leukotrienes
Inducible Nitric Oxide Synthase (iNOS) → Nitric Oxide (NO)
Tissue Necrosis Factor (TNFα)
*Cell Adhesion Molecules (CAMs)

Step 4. Health conditions created by prolonged exposure to the above chemicals.

• Pain
• Inflammation (edema, swelling)
• Asthma and Hypersensitivity to Potential Allergens
• Cardiovascular disease
• Thrombosis (blood clots)
• Insulin resistance
• Autoimmune disease (Hashimoto’s, Lupus…)
• Rheumatoid disease
• Neurodegeneration (Alzheimer’s disease, Parkinson’s disease)
• Cancer

Step 5. IL-1, PGE2, reactive oxygen species and oxidative stress, TNFα and CRP created because of the above health conditions, facilitate the continued activation of NF-κB.

Step 6. In order to break the above inflammatory cycle, stressors need to be removed,  severely reduced or reversed. One of the easiest steps that can be taken is to eliminate table sugar, vegetable oil and hydrogenated fats out of the diet and add in Omega 3 fatty acids, in the form of fish and flax seed oil. Next, is to reduce animal protein (red meat) consumption to 6 ounces per day and add-in nutrient dense foods like: vegetables, fruits, nuts, seeds and beans. Increasing antioxidants and anti-inflammatory nutrients is a cost effective measure that will pay dividends in the form of less sickness, disease and an increased life-span. Here are a few of my favorite nutrients that support  anti-inflammatory processes: molecularly distilled fish oil, natural vitamin C, chlorella, vitamin E, selenium, NAC, resveratrol, grape seed extract and curcumin. Here are a few of my favorite antioxidant rich (anti-inflammatory) fruits and vegetables: red bell pepper, tomato, cilantro, spinach, red cabbage, onion, garlic, strawberries, blueberries and red raspberries. Lastly, engaging in mild-moderate exercise (3 days/week), taking a broad-spectrum, enteric-coated probiotic and 4,000 – 10,000 IU’s of vitamin D3 per day, will round-out any  anti-inflammatory protocol.

Step 7. Proactive diagnostic blood tests to determine the presence and level of inflammation within the body, as well as tests that will determine risk for development of cardiovascular disease (heart attack) or cerebrovascular disease (stroke). Any of these tests can be ordered by calling our office 714-639-4360

• C-Reactive Protein (CRP) – High Sensitive (Cardiac)
• Homocysteine
• Fibrinogen
• Hemoglobin A1C
• IL-6 – Interleukin – 6
• Sed Rate (ESR)
• Lipid Panel
• D,25 Hydroxycalciferol – Vitamin D

*Atherosclerosis is strongly considered to be a low-grade inflammatory response of the arterial wall to a variety of stimuli in all aspects of coronary syndromes, from the pathogenesis of atherosclerosis to plaque rupture and myocardial cell death. In each of these steps, infiltration of cardiovascular tissue by inflammatory cells is evident and is regulated by a number of cytokines (IL-6 and CRP) and cell adhesion molecules.  Maseri A. N Engl J Med. 1997;336:1014-1016

Monolaurin - Monolauroylglycerin - Glycerol monolaurate

Lauric acid is a 12-carbon medium chain fatty acid (MCFA) found naturally in human breast milk (6.2% of total fat) and coconut oil (47.5% by weight). Lauric acid was originally discovered when microbiologists studied human breast milk to determine the protective (anti-viral and anti-bacterial) substances which protected infants from microbial infections. Other fatty acids were also found to have antimicrobial actions but lauric acid was found to be the most active.

The esterification of lauric acid, that naturally occurs in our body, yields an amazing compound known as monolaurin (glycerol monolaurate). Monolaurin is a non-ionic surfactant¹, which possesses an even greater anti-viral and anti-bacterial activity than its precursor, lauric acid. Monolaurin, when given orally, at therapeutic doses between 2,500 – 4,500 mg/day is generally well tolerated, with loose bowels as the only negative concern. Monolaurin has been studied at medical research centers, including the Center for Disease Control (CDC), because of its high antimicrobial (anti-viral, anti-bacterial, anti-fungal, anti-yeast and anti-protozoal) activity. These studies have provided information about the anti-viral and anti-bacterial mechanisms of monolaurin. Monolaurin was found to be effective against certain Lipid Coated Bacteria (LCBs) and Lipid Coated Viruses (LCVs) – enveloped DNA and RNA viruses.

¹Surfactant: Surfactants are compounds that lower the surface tension of a liquid, allowing easier spreading, and lowering of the interfacial tension between two liquids, or between a liquid and a solid. Surfactants may act as: detergents, wetting agents, emulsifiers, foaming agents, and dispersants.

Lipid Coated Virus

HIV-1, Influenza virus, paramyxoviruses, rubeola virus, bronchitis virus, and the herpes family of viruses (Epstein-Barr, cytomegalo, zoster, vericella-zoster and herpes type I and II). Sadly, monolaurin had no effect on diseases caused by non-enveloped viruses such as polio virus, coxsackie virus, encephalomyocarditis virus, rhinovirus, and rotaviruses.

Lipid Coated Bacteria

Helicobacter pylori (H. pylori),  Staphylococcus aureus and Streptococcus agalactiae.

The anti-viral and anti-bacterial action attributed to monolaurin is that of solubilizing the lipids and phospholipids in the protective envelope of these particular infective agents causing the disintegration of the lipid envelope. Recent publications have shown that monolaurin and lauric acid inhibit the replication of viruses by interrupting the communication and binding of virus to host cells and thus preventing the uncoating of viruses necessary for replication and infection. Other studies have shown that monolaurin is able to remove all measurable infectivity by directly disintegrating the protective bacterial and viral lipid envelop. Binding of monolaurin to the viral envelop also makes the virus more susceptible to degradation by host defenses, heat, or ultraviolet light.

Microorganisms Inactivated by Monolaurin

Ecological Formulas Monolaurin (600 mg’s – 90 caps) $34.00 or Lauricidin® The Original Monolaurin 227gr $40.00 Call to Purchase 714-639-4360

The discovery that bacteria are able to communicate with each other changed our general perception of many single, simple organisms inhabiting our world. Instead of language, bacteria use signaling molecules which are released into the environment. As well as releasing the signaling molecules, bacteria are also able to measure the number (concentration) of the molecules within a population. Nowadays we use the term ‘Quorum Sensing’ (QS) to describe the phenomenon whereby the accumulation of signaling molecules enable a single cell to sense the number of bacteria (cell density). In the natural environment, there are many different bacteria living together which use various classes of signaling molecules. As they employ different languages they cannot necessarily talk to all other bacteria. Today, several quorum sensing systems are intensively studied in various organisms such as marine bacteria and several pathogenic bacteria.

Quorum Sensing & Biofilm Formation

Why do bacteria talk to each other?

(QS) enables bacteria to co-ordinate their behavior. As environmental conditions often change rapidly, bacteria need to respond quickly in order to survive. These responses include adaptation to availability of nutrients, defense against other microorganisms (biofilm formation) which may compete for the same nutrients and the avoidance of toxic compounds (biofilm formation) potentially dangerous for the bacteria. It is very important for pathogenic bacteria during infection of a host (e.g. humans, other animals or plants) to co-ordinate their virulence in order to escape the immune response of the host in order to be able to establish a successful infection. The University of Nottingham Quorum Sensing Research Group

From Dr. Ettinger’s Biofilm Protocol for Lyme and Gut Pathogens: Pathogenic bacteria known to reside in biofilms include: Borrelia burgdorferi, Escherichia coli, Candida albicans, Clostridium difficile, Clostridium perfringens, Helicobacter pylori, Klebsiella pneumoniae, Legionella pneumophila, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, and Vibrio cholerae. The number of human diseases shown to be associated with biofilms is expanding and includes chronic bacterial prostatitis, chronic rhinosinusitis, cystic fibrosis pneumonia, infective endocarditis, periodontitis, recurrent otitis media, and virtually all device and implant related infections. Strong evidence is also beginning to emerge for an etiologic role of pathogenic mucosal biofilms in gastrointestinal diseases, such as Irritable Bowel Disorders: Crohn’s disease and ulcerative colitis.

Excessive negative stress has become, over the last 25 years, as much a part of our daily lives as eating and sleeping. Excessive negative stress can be physical (chronic illness, lack of exercise, over-exercising, etc.), emotional (losing a job, winning the lottery, etc.), or chemical (bad diet, drugs, medicines, alcohol, pesticides, etc.).

Excessive negative stress is most likely to be the initial predisposing factor in the cause and prolongation of a disease or illness. Again, the greater the exposure and duration to these negative stressors the greater the susceptibility to acute or chronic illness or body dysfunction.

It is also important to note that not all stress is bad or has negative effects on our bodies. Some types of stress are actually beneficial for us and can increase our immune function, overall strength, emotional well being and longevity. The most beneficial form of stress is exercise, especially swimming and weight training. This type of stress can, in moderation, even negate some of the excessive negative stress’ we face.

When our bodies are exposed to any form of negative stress our organ systems innately responds with what is known as the “fight-flight response”. During this stress induced response, many different and potentially damaging physiological reactions occur. During the initial phase, which can last for seconds to years, digestion and absorption of nutrients are greatly reduced; body tissues begin to break down; the immune system is suppressed; our heart begins to overload and the aging process is significantly accelerated. These are but a sampling of what does occur.

Individuals who are exposed to excessive negative stress may also develop a condition known as adrenal exhaustion. This condition occurs when the adrenal glands work beyond their normal capacity thus eventually shutting down (no more Cortisol, a hormone, being produced). Adrenal exhaustion presents with symptoms similar to those found in patients with chronic fatigue syndrome (CFS), hypothyroidism, fibromyalgia and hypoglycemia (see table 1).

Table 1

An integrative medicine approach for the treatment of adrenal exhaustion or the effects excessive negative stress are identical. First, it’s crucial to reduce or eliminate as many negative stressors (individual stress factors) as possible.

Second, get plenty of rest, at least 8 hours, starting before midnight; engage in regular, but not excessive, exercise; greatly reduce your carbohydrates intake (bread, sweets, pasta, corn and potatoes); increase protein intake; increase fibrous vegetable intake; drink eight glasses of filtered water daily, and perform deep breathing exercises every morning and evening.

Third, nutritional and/or pharmacological support. To determine pharmacological support we must first determine thyroid and adrenal function via blood tests (thyroid panel including both thyroid antibodies and rT3), and (DHEA, cortisol and progesterone) levels. Nutritional support can be in the form of DHEA and/or Pregnenolone (10 – 50 mg daily) and/or glycerrhizin (25 – 100 mg daily – preferably in the morning). These two supplements safely support proper adrenal hormone levels. DMG (vitamin B15) and Siberian Ginseng have both been shown in Russian clinical studies to help regulate the hypothalamus, pituitary, adrenal axis. Pantothenic acid (vitamin B5) is the initial precursor, along with our LDL cholesterol to our adrenal hormones, whole C complex (Ester-C w/bioflavonoids) is an antioxidant for the adrenal and the highest concentration of vitamin C out side of the spinal chord and brain is found in the adrenal; and potassium are essential for proper adrenal gland function. Adrenal glandular preparations (Whole Desiccated Adrenal (short-term), Drenamin and Drenatrophin PMG) from Standard Process Inc. will also help to support the adrenal glands. These are but a few options that I have used to support the stress response of the body.

Additionally, antioxidants and supportive products like: CoQ10, glutathione, NAC, vitamin E, bioflavonoids, alpha lipoic acid, SAMe, Gabatone (Apex Energetics), L-tryptophan, magnesium and MSM, will also help reduce the overall oxidative burden placed on the body by the excessive negative stress.

With the above knowledge the road to well being and health is now a much shorter one.

H1N1 Virus

To make a long story short – My family and I are not going to be vaccinated for the “seasonal flu” or “H1N1 Flu virus”. Instead, we are taking daily, my Proactive, Immune-Boosting Nutritional Protocol.   In 21 years as an holistic  and integrative medicine practitioner, I have never taken a sick day.  I’ve had a runny nose and a scratchy throat but that’s it.  In that same time span, I’ve been sneezed on and coughed on by hundreds of patients with the flu, pneumonia, bronchitis, sinusitis and colds.  The point is – I know what to do to stay well.

With the flu season moving into high gear, I am not worried or scared about the current Swine Flu pandemic – I am getting prepared, but not scared.  My family and I are taking proactive, preventative steps that I know will enable our innate immune system to defend itself against the bacteria’s and viruses we will be exposed to.  I am not saying that by taking these preventative steps, my family and I will be immune super-beings, capable of eating Swine Flu tacos for dinner.  What I am saying is that, with 100% confidence, we will possess the capability to defend ourselves, since all will be well within our bodies.  At a minimum we will possess the capability to minimize the effects/symptoms after exposure to a contagion (bacteria/virus).  The key here is to minimize the impact.

My Proactive, Immune Boosting Nutritional Protocol:

1. Liquid Vitamin D Forte – Biotics Reseach [Adults 6,000 IU's and children above the age of 5 can take 2,000 IU's per day]
2. Probiotic Defense or GR8 (multi-strain probiotic) – both by Now Foods [2 doses of either per day. I prefer the Probiotic Defense Powder]
3. Chewable, buffered Vitamin C w/rutin [I like Now Foods brand. 2-4 per day will do.]

(None of the above products are not intended to diagnose, treat, cure or prevent any disease.)

There are many other preventative products out there, but this, as far as I am concerned, is the best and least expensive, Immune Boosting Protocol available.

FYI: Avoid products containing sucrose or high fructose con syrup. Processed sugar in an immunosuppressant – Not a good thing. Also wash your hands, engage in moderate exercise, drink plenty of water and get at least 8 hour of sleep each night.

To purchase any of these products, please give us a call at (714) 639-4360. We can ship anywhere in the US and Canada.

Applicable References:

• Arch Intern Med. 2009 Feb 23;169(4):384-90.

Association between serum 25-hydroxyvitamin D level and upper respiratory tract infection in the Third National Health and Nutrition Examination Survey. Ginde AA, Mansbach JM, Camargo CA Jr. Emergency Medicine Network, Massachusetts General Hospital, 326 Cambridge Street, Boston, MA 02114, USA.

“Recent studies suggest a role for vitamin D in innate immunity, including the prevention of respiratory tract infections (RTIs).”

• Eur J Clin Nutr. 2009 Apr;63(4):473-7. Epub 2007 Nov 21.

Association of subclinical vitamin D deficiency in newborns with acute lower respiratory infection and their mothers.
Karatekin G, Kaya A, Salihoğlu O, Balci H, Nuhoğlu A. Department of Neonatology, Sişli Etfal Teaching and Research Hospital, Istanbul, Turkey. gunerkaratekin@yahoo.com

CONCLUSIONS: Our findings suggest that newborns with subclinical vitamin D deficiency may have an increased risk of suffering from ALRI. The strong positive correlation between newborns’ and mothers’ 25(OH)D concentrations shows that adequate vitamin D supplementation of mothers should be emphasized during pregnancy especially in winter months.

First a little background on biofilm:

Fig. 1: The biofilm life cycle. 1: individual cells populate the surface. 2: extracellular polymeric substance (EPS) is produced and attachment becomes irreversible. 3 & 4: biofilm architecture develops and matures. 5: single cells are released from the biofilm. Related Post – Biofilm Basics and Quorum Sensing and Biofilm

This is an excerpt from a Klaire Labs product monograph which is a basic primer on the topic (My additions are in RED) The National Institutes of Health estimates that 60% of all human infections and 80% of refractory infections (def. unresponsive to medical treatment) are attributable to biofilm colonies.  I have seen this, most commonly, in cases I’ve worked-up, where the pathogen is: Chlamydia pneumoniae, Pseudomonas aeruginosa, Helicobacter pylori, [Lyme disease - Borrelia burgdorferi] and Candida albicans.

• The protection conferred upon microorganisms by biofilm allows them to achieve a high level of antibiotic resistance, stealth and invisibility.

• Biofilm not only provide a physical barrier to antimicrobial agents (pharmaceutical antibiotics) and host antibodies, but facilitate the exchange of antibiotic-resistant genetic material between organisms and may contain antibiotic-degrading (hydrolysing) enzymes such as b-lactamase, effectively neutralizing incoming antibiotic (b-lactam antibiotics) molecules.

• In fact, biofilm communities can be 1000 times more resistant to antibiotics than free-floating bacteria.

• The decreased growth rate of sessile microorganisms (def. Permanently attached to a substrate; not free to move about; “an attached oyster”) also reduces their antibiotic susceptibility as most antimicrobial agents require rapid cell growth in order to effectively kill or inhibit the microbes.  Biofilm thus render pathogenic microorganisms enormously difficult to eradicate, and can almost single-handedly contribute to localized or systemic inflammatory reactions and delayed wound healing.

• Depending on the type of biofilm, one or more species of pathogens may be found embedded in the extracellular polymeric substance (def. Composed primarily of polysaccharides and can either stay attached to the cell’s outer surface, or be secreted into its growth medium).  Bacterial extracellular polymeric substance (EPS) maybe a carrier of, or may have heavy metals embedded in them, thus the indication for chelation w/EDTA. EDTA, ethylenediaminetetraacetic acid, is a chelating agent used to lower one’s body burden of heavy metals).

Pathogenic bacterial known to reside in biofilms include, but are not limited to: Borrelia burgdorferi (Lyme bacteria), Escherichia coli, Candida albicans (yeast and fungal mutation), Clostridium difficile, Clostridium perfringens, Helicobacter pylori, Klebsiella pneumoniae, Legionella pneumophila, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, and Vibrio cholerae. The number of human diseases shown to be associated with biofilms is ever expanding and includes: chronic bacterial prostatitis, chronic rhinosinusitis (chronic sinus infections), cystic fibrosis pneumonia, infective endocarditis, periodontitis, recurrent otitis media, and virtually all device and implant related infections.  Strong evidence is also beginning to emerge for an etiologic (causative) role of pathogenic mucosal biofilm in gastrointestinal diseases, such as Irritable Bowel Disorders (IBS): Crohn’s disease and ulcerative colitis.

S. aureus biofilm

Dr. Marcus Ettinger’s Biofilm Protocol – Only the eradication phase is presented here.  There is a pre,  post and toxin reduction step as well.  You can get help with any of these steps – HERE

A. Products (mandatory products in red). These are ONLY the basics. Additional nutraceuticals may be needed, based on each individuals unique situation.

1. Monolaurin [AKA Glyceryl laurate or glycerol monolaurate] (monolaurin information) or Lauricidin
2. Nutiva Extra-Virgin Coconut Oil (42-52% Medium Chain Fatty Acids [MCFA], lauric acid, by volume)
3. Nattokinase (a potent fibrinolytic enzyme) I like this better than Lumbrokinase.
4. InterFase Plus™ (broad-spectrum enzyme formula w/EDTA)
5. Serrapeptase (a potent fibrinolytic enzyme)
6. Vitamin C (ascorbic acid – Not buffered, as most of these contain metals)
7. NAC (N-Acetyl-Cysteine)
8. Lactoferrin (specifically Nutricillin by Ecological Formulas) Dr. Anju Usman of Illinois states, “Our bodies make proteins, transferrin and lactoferrin, which mop up iron and block the ability of biofilm to form,” she said. “But pathogenic bacteria secrete iron chelators to snatch up iron and thus compete with the transferrin and lactoferrin for what they need to survive.”

B. Avoid supplemental forms of: magnesium, iron and calcium during the biofilm protocol, as they may contribute to  biofilm formation or decrease the effectiveness of the biofilm protocol.

C. Take a broad-spectrum probiotic and prebiotic.  I like the combination of Now Foods brand Probiotic-10 and their Probiotic Defense Powder (contains gluten).  These products will help to crowd out the bad bacteria, and also help disrupt and replace biofilm colonies along the mucus membrane.

D. Specific additions based on condition (not a complete list):

1. Candida albicans – SF722* (10-Undecenoic Acid  50 mg) Thorne Research. This is as close as you can get to a medication and still be a natural substance.  There are a few chat rooms blasting this product, based on who knows what – can’t make everyone happy.  I’ve used SF722 for over 15 years and it is amazing – never a problem!  *Do not take SF722 if you are allergic to fish. There are many other amazing products that can be added to complement the SF722. It’s really a matter of how many pills someone wants/doesn’t want to take per day or the severity of one’s condition, that will determine, if or which, additional products will be added. If the Candida albicans overgrowth is severe, has not responded to holistic methods or has mutated into its more virulent hyphal form/fungal infection (nails, underarms, groin or skin); Diflucan (fluconazole), a prescription medication, is my personal preference, but Nizarol (ketoconazol) can also be used. In Azole-resistant Candida albicans, lactoferrin must be added to either medication in order to increase their effectiveness. There is a certain B vitamin, mineral and amino acid that possesses synergistic qualities and I find them indispensable when taking Diflucan (fluconazole), Nizarol (ketoconazole) or for supporting candida die-off symptoms.
2. Chlamydia pneumonia, Klebsiella pneumoniae or Pseudomonas aeruginosa – Pneumotrophin PMG by Standard Process, Inc. How it works.  I use this because it helps direct the body’s attention and healing efforts to the lung, where it’s needed most. Apex Energetics, H-PLR is also a mandatory addition. I also like to use OOrganik-15™ and Pneuma-Zyme™ by Biotics Research with some of my patients who manifest asthma, a chronic cough and/or emphysema like symptoms.     
3. H. pylori – Complete write-up on another post.
4. Chronic bacterial prostatitis – Quercitin (600mg’s) and Bromelain (200mg’s) combination by Now Foods. Decreases inflammation and oxidant stress in the prostate while increasing local concentrations of beta-endorphins. Apex Energetics, H-PLR is also a mandatory addition.

E. Certain dietary restrictions and additions will need to be taken. These are determined on a case by case basis.

Important Note:

All dosages will be provided if you purchase some or all of your “biofilm protocol” products through my office. I truly do want to help all who are interested, but it’s finally gotten to the point where too many people want free advice and an increasing amount of my time, and then buy all of their products elsewhere. I am a firm believer in fair exchange and I feel I have done that by providing the information in this post.

I also offer tailor made protocols for your individual situation, please contact our office for product prices and distance patient information (714) 639-4360. 

 

 

 

Biofilm testing is also available through Fry Laboratories. Fry Laboratories, L.L.C. is an independent clinical diagnostic and research laboratory located in Scottsdale, Arizona. We are committed to understanding chronic diseases and contributing to their cure through advancements in diagnostics and basic science research with emphasis on chronic inflammatory diseases, vector-borne diseases, and their intersection. Our clinical diagnostic laboratory offers general and targeted immunology services in conjunction with standard and cutting edge infectious disease detection and identification technologies. Our signature services include microscopy for visual identification and quantification of a wide range of blood-borne pathogens, co-infection serology, biofilm detection, and genus wide molecular detection technology with sequencing for individualized species and/or strain identification. We participate in both CAP and API quality control programs and provide worldwide testing service.

Diseases of Interest: Chronic Fatigue Syndrome, Fibromyalgia, Gulf War Veterans Illness, Chronic Lyme Disease, ALS (Lou Gehrig’s Disease), Parkinson’s Disease, Multiple Sclerosis, Autism, Lupus, Ulcerative Colitis, Scleroderma, Rheumatoid Arthritis, Osteoarthritis, Crohn’s Disease.

Infections of Interest: Borrelia (Lyme), Babesia, Bartonella, Anaplasma, Ehrlichia, Q-Fever (Coxiella), Toxoplasma, Rickettsia, Plasmodium, XMRV

Important: This post is not a substitute for medical advise or treatment and is for informational purposes only. Please consult with a physician before starting any nutritional or biofilm protocol on your own.

My theory has developed over a 20 year period of time and holds credibility as a corollary to the long-standing law in anatomy and physiology known as – Wollf’s law.  Wolff’s law, in short, states that, “bone remodels according to the physical stress placed upon it.”  As an example: a pitcher’s, pitching arm (humerus, radius and ulna) will be denser than his non-pitching arm.  The opposite of this is, if we lived in outer-space our bones would dissolve (osteoporosis) because of the lack of gravity; no weight-bearing load on the bones.  All of the calcium supplementation, estrogen replacement (for women), and Fosimax (medication) in the world would not prevent this process from happening.

My theory takes Wollf’s law to the next level and states, “Our entire body remodels according to the stress placed upon it: invisibly, physically, emotionally and chemically.” All four of these stressors or outside influences/forces, will directly affect the remodeling of every cell, tissue and physiological function of the body.  This means that our inward and outward appearance and physiological state is in direct correlation to the (way, what and/or how) we sleep, eat, drink, act, exercise, think, deal with stress, or are exposed to stress’ or outside forces.

Physical stress makes-up the largest portion of the overall stressors we will be subjected to on a daily basis. The foods we eat and how often, our exercise level or lack of exercise, chronic postures (work, driving and sleep), chemicals, pesticides, excessive alcohol, medicines, drugs and our body’s own waste products, all influence how we remodel.

If we ingest all of the essential nutrients that our body requires, with all other factors aside, our body will remodel properly. The opposite can be said for eating devitalized food, such as fast food, boxed or canned food; as well as being exposed to drugs, medicines, alcohol, pesticides, toxins, etc… Examples: If we increase protein and weight bearing exercise, an increase in muscle mass and strength will be the result. If we increase fats and/or carbohydrates, combined with a lack of physical exercise, an increase in body fat, blood sugar, blood fats and blood pressure will be the result. This is called: diabetes, obesity, high cholesterol and hypertension. Exposure to toxins, pesticides, molds, drugs or excessive alcohol, may lead to cancer, ADHD and many other diseases.

Emotional stress placed on the body will have similar effects. If we feel or are exposed to happiness, joy or generosity, all good stress’ will facilitate a positive remodeling (ex. neurotransmitter and hormone production). If we feel or are exposed to anger, expressed or unexpressed resentment, blame or shame, all negative emotions, this will facilitate our body to remodel in a non-optimum way (increased inflammation on decreased endocrine and brain function – Parkinson’s and Alzheimer’s).  The negative remodeling can lead to everything we don’t want to have our body experience, such as cancer, autoimmune disease and body degeneration. The positive remodeling will help increase the immune system and overall strength of the body, leading to a longer, healthier and more vibrant life.

Invisible forces (electromagnetic waves [radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays], gravity and ionizing radiation) are constantly present and can affect us as much as the overt chemical, emotional and physical forces do. The invisible forces, most of the time, actually precede and initiate the physical, emotional or chemical stress’ that causes the remodeling of our body’s cells or, more specifically, the cell membranes.

It’s easy to see from this, that everything we do or don’t do, and everything that is done to us, sometimes unknowingly, affects how our body remodels. This means how we look and how we functions.  The point I am trying to make here is that we can at least be “cause” of over a very large part of this and be responsible for what we do on a daily basis (eat, exercise, rest…) and what we allow around us. If we do not learn from this then we can go through life at “effect” like a stick floating down a river, out of control. By ignoring this theory, negative remodeling will be the outcome and all the negative consequences that come with it. The choice is ours.

©06 January 2009 Marcus Ettinger and AdvancedHealing.Com. All rights reserved (no portion of this may be re-printed or used without permission)