Important Note: To receive biofilm protocol information, coaching on a biofilm protocol, or support for H. pylori eradication.., you will need to become a distance patient – New Patient Forms. I truly do want to help any and all who are interested, but it’s finally gotten to the point where far too many people want free advice, treatment plans, personalized protocols, etc. I firmly believe in fair exchange, and I feel I have done so by providing the information in this post.
I want to help you, and I can create a tailor-made protocol for your situation. Please fill out the distance patient application (link above). If you have a specific question about the program, please call me first at 714-639-4360.
Table of Contents
- Dr. Ettinger’s Biofilm Protocol for Lyme and Gut Pathogens
- First, a little background on what is biofilm.
- Related Biofilm Protocol Posts
- Biofilm and Chronic Infection: Key Points (my additions and comments are in RED)
- Pathogenic bacteria that are known to form biofilm include, but are not limited to:
- The number of human diseases associated with biofilm is ever-expanding and includes:
- Dr. Marcus Ettinger’s Biofilm Protocol
- Biofilm Busting (Disrupting) Nutraceuticals
- Current H. pylori Protocol and December 13, 2016 Update (Pylopass)
- September 26, 2016 Update – IMPORTANT
- Biofilm Protocol Avoid or Add?
- Probiotics
- Beneficial Yeast
- Specific additions based on condition (Not a complete list)
- Diet
- Testing
- Biofilm Protocol Tips for Bacterial and Yeast Infections
- Final Words
- Research
Dr. Ettinger’s Biofilm Protocol for Lyme and Gut Pathogens
Copyright © Marcus S. Ettinger, DC 2009-2024
A specific question has been asked a lot lately: What is my protocol for busting through bacterial biofilm? This question has mostly come from people diagnosed with or those who think they may have H. pylori bacterial infection or Lyme disease. For additional information on the link between Lyme and biofilm, please check out this 2008 presentation: Biofilms of Borrelia burgdorferi And Clinical Implications for Chronic borreliosis by Alan B. MacDonald, MD. For information on Lyme Disease and Herpes Virus Connection. The reason that I’ve put this “biofilm busting/disrupting protocol” post together is based on this one fact. The day I discovered how to have an effect on bacterial biofilm or its communication within the human body was the day that chronic conditions, from the sinus to the prostate, were no longer a ‘project’ to handle. I hope the data below is helpful to you in your search for information or health restoration.
First, a little background on what biofilm is:
Figure 1: The life cycle of biofilm. 1: individual bacterial cells populate a surface. 2: The extracellular polymeric substance (EPS) is produced, and attachment becomes irreversible. 3 & 4: Biofilm architecture develops and matures. 5: Single planktonic cells (planktonic – meaning free-floating) are released from the biofilm.
Tuned-Down Biofilm Definition: A complex structure adhering to surfaces that are regularly in contact with water, consisting of colonies of bacteria and usually other microorganisms such as yeasts, fungi, and protozoa that secrete a mucilaginous protective coating in which they are encased. Biofilms can form on solid or liquid surfaces as well as on soft tissue in living organisms and are typically resistant to conventional methods of disinfection. Dental plaque, the slimy coating that fouls pipes and tanks, and algal mats on bodies of water are examples of biofilms. While biofilms are generally pathogenic in the body, causing such diseases as cystic fibrosis and otitis media, they can be used beneficially in treating sewage, industrial waste, and contaminated soil.
The American Heritage® Science Dictionary
Tuned-up Biofilm Definition: Direct observations have clearly shown that biofilm bacteria predominate, numerically and metabolically, in virtually all nutrient-sufficient ecosystems. Therefore, these sessile organisms predominate in most of the environmental, industrial, and medical problems and processes of interest to microbiologists. If biofilm bacteria were simply planktonic cells that had adhered to a surface, this revelation would be unimportant, but they are demonstrably and profoundly different. We first noted that biofilm cells are at least 500 times more resistant to antibacterial agents. Now, we have discovered that adhesion triggers the expression of a sigma factor that derepresses many genes so that biofilm cells are clearly phenotypically distinct from their planktonic counterparts. Each biofilm bacterium lives in a customized microniche in a complex microbial community that has primitive homeostasis, a primitive circulatory system, and metabolic cooperativity, and each of these sessile cells reacts to its special environment so that it differs fundamentally from a planktonic cell of the same species. Lappin-Scott HM, et al. Microbial biofilms. Annu Rev Microbiol. 1995;49:711-45.
Related Biofilm Protocol Posts
- Biofilm Basics.
- Bill Costerton – The “Father” of Biofilms, has some great information on a variety of conditions.
- Quorum Sensing and Biofilm
- Autoinducers, Biofilm, Bacterial Density, and Quorum Sensing
- Princeton researchers have, for the first time, revealed the mechanism of how bacteria, cell by cell, create protective masses called biofilm.
- Biofilm Protocol Basics For Bacterial And Yeast Infections
Pic. A simulation of the bacteria Vibrio cholerae forming a biofilm, with each slightly curved, rod-shaped unit indicating individual bacteria. The architecture shows vertically oriented bacteria at the biofilm’s center and horizontally oriented bacteria at the bottom, adhering to the surface upon which the mass grows. Credit: Image courtesy of Bonnie Bassler, Howard Stone, Ned Wingreen, and Jing Yan
Biofilm and Chronic Infection: Key Points (my additions and comments are in RED)
Excerpts from a Klaire Labs product monograph, which is a basic primer on the topic of bacterial biofilm.) The National Institutes of Health (NIH) estimates that 60% of all human infections and 80% of refractory infections (def. unresponsive to medical treatment) are attributable to biofilm colonies. I have seen this, most commonly in cases I’ve worked up where the pathogen is: Chlamydia pneumoniae, Pseudomonas aeruginosa, Helicobacter pylori, [Lyme disease – Borrelia burgdorferi], and Candida albicans.
– The protection conferred upon microorganisms (primarily gram-negative bacteria) by biofilm allows them to achieve a high level of antibiotic resistance, increased virulence, stealth, and invisibility.
– Biofilm not only provides a physical barrier to antimicrobial agents (pharmaceutical antibiotics) and host antibodies but facilitates the exchange of antibiotic-resistant genetic material between organisms and may contain antibiotic-degrading (hydrolyzing) enzymes such as b-lactamase, effectively neutralizing incoming antibiotic (b-lactam antibiotics) molecules.
– In fact, biofilm communities can be 400-1000 times more resistant to antibiotics than free-floating planktonic bacteria.
– There may be 10, 20, 30, 40, 50, 60 different types of bacteria within a biofilm, especially in infected wounds, post-surgical.
– The decreased growth rate of sessile microorganisms (def. Permanently attached to a substrate; not free to move about; “an attached oyster”) also reduces their antibiotic susceptibility as most antimicrobial agents require rapid cell growth in order to kill or inhibit the microbes effectively. Biofilm thus renders pathogenic microorganisms enormously difficult to eradicate and can almost single-handedly contribute to localized or systemic inflammatory reactions, autoimmune reactions, and delayed wound healing.
– “…. Once established, however, biofilm infections persist. They are rarely resolved by host defense mechanisms, even in individuals with healthy innate and adaptive immune reactions. Active host responses, such as invading neutrophils (the most abundant type of white blood cell in mammals and forms an essential part of the innate (inborn; natural) immune system), can even be detrimental since those cells can cause collateral damage to neighboring healthy host tissue. Biofilm infections respond only transiently to antibiotic therapy.” From Biofilms Hypertext, Chapter 4 Biofilms in Health and Medicine contributing author James Garth, PhD
– Depending on the type of biofilm, one or more species of pathogens may be found embedded in the extracellular polymeric substance (def. Composed primarily of polysaccharides and can either stay attached to the cell’s outer surface or be secreted into its growth medium). Bacterial extracellular polymeric substances (EPS) may be a carrier of or may have heavy metals embedded in them, especially iron, thus the indication for chelation w/EDTA. EDTA, ethylenediaminetetraacetic acid, is a chelating agent used to lower one’s body burden of heavy metals). This is why Interfase Plus and lactoferrin are so useful.
Pathogenic bacteria that are known to form biofilm include, but are not limited to:
- Borrelia burgdorferi (Lyme bacteria)
- Escherichia coli (linked to colon cancer, bladder, and other infections)
- Bacteroides fragilis (linked to colon cancer)
- Candida albicans (yeast and fungal mutation)
- Clostridium difficile (the most common cause of GI infection and a growing epidemic)
- Clostridium perfringens
- Helicobacter pylori (linked to ulcers, gastritis, and stomach cancer)
- Klebsiella pneumoniae
- Legionella pneumophila
- Listeria monocytogenes
- Pseudomonas aeruginosa
- Salmonella typhimurium
- Staphylococcus aureus
- Staphylococcus epidermidis
- Vibrio cholerae
Chlamydophila species such as Chlamydia pneumoniae don’t form a biofilm, as they are intercellular, but may somehow get accidentally caught up in them before entering a host cell. Here is a good video on Chlamydia and biofilm (Video [biofilm section 7:45 min. mark] – Dr. Wilmore Webley on C. pneumoniae & Biofilms).
The number of human diseases associated with biofilm is ever-expanding and includes:
- Chronic bacterial prostatitis (chronic prostate infection)
- Chronic rhinosinusitis (chronic sinus infections)
- Cystic fibrosis pneumonia
- Infective endocarditis (infection of the thin, smooth membrane that lines the inside of the chambers of the heart and forms the surface of the valves)
- Periodontitis (also known as pyorrhea, is a serious gum infection)
- Recurrent otitis media (recurrent ear infection)
- Colon cancer – Bacterial Biofilms Linked To Colon Cancer and Gut Microbes Combine to Cause Colon Cancer
- Virtually all device and surgical implant-related infections.
- Strong evidence is also beginning to emerge for an etiologic (causative) role of pathogenic mucosal biofilm in gastrointestinal diseases, such as Irritable Bowel Disease (IBD), Crohn’s disease, and ulcerative colitis. Leaky gut and IBS may also have biofilm as a contributing factor.
Figure 2: S. aureus biofilm
Dr. Marcus Ettinger’s Biofilm Protocol
You can get help with any of these steps by going to my Distance Client Program.
PLEASE, PLEASE, DO NOT SELF-TREAT! —— READ MY UPDATED UPDATE BELOW FOR MY EXPLANATION.
Biofilm Busting (Disrupting) Nutraceuticals
This is not an exhaustive list of the nutraceuticals that can be used as biofilm disruptors. Not all of these products will be or should be used at the same time. Additional nutraceuticals may be needed based on each individual’s unique situation, genetics, and epigenetic factors. The key to a successful outcome is twofold. First, it’s building a comprehensive protocol of both biofilm-disrupting and antimicrobial agents. The second is to make sure that all of the protocol’s ingredients are taken at therapeutic levels. If either of these key points is out, both money and time can be wasted.
- Monolaurin or Lauricidin [AKA Glyceryl laurate or glycerol monolaurate] Read more – Antiviral and Antibacterial Actions of Monolaurin and Lauric Acid
- Nattokinase (a potent oral fibrinolytic enzyme supplement). Some prefer Boluoke Lumbrokinase.
- InterFase Plus™ (a broad-spectrum enzyme formula with EDTA) – Use only under a doctor’s supervision.
- InterFase™ (a broad-spectrum enzyme formula without EDTA)
- NAC (N-Acetyl-Cysteine) Mucolytic enzyme N-acetylcysteine (NAC) is a precursor of glutathione and a potent antioxidant. Effective against biofilm on prosthetic devices, in vitro biofilm, and chronic respiratory infections (1, 2, 3, 4, 5 ), NAC is a powerful molecule able to destroy bacterial biofilm (6).
- Curcumin 95% Natural Turmeric Extract Powder (Curcuma longa)
- Lactoferrin Dr. Anju Usman, “Our bodies make proteins, transferrin, and lactoferrin, which mop up iron and block the ability of biofilm to form,” she said. “But pathogenic bacteria secrete iron chelators to snatch up iron and thus compete with the transferrin and lactoferrin for what they need to survive.”
- Xylitol (sugar alcohol): Do not use it if you have IBS or IBD.
- Nutiva Extra-Virgin Coconut Oil (42-52% Medium Chain Fatty Acids [MCFA], lauric acid, by volume)
- Serrapeptase (a potent oral fibrinolytic enzyme supplement)
- Wobenzyme N
- Pepsin
- Guaifenesin (Maximum-Strength Mucinex)
- Neem oil
- Reishi Mushroom
- BFB-1™ & BFB-2™ (essential oil preparations)
- Smilax Officinalis
- Carbonized Bamboo
- Bladderwrack
- Specific Plant Tannins and/or Polyphenols based on individual need
- Specific types of Soluble Fiber and/or Insoluble Fiber, based on individual need and if there are no contraindications for their use.
- Specific Plant-Based Enzymes, based on individual need
- Transdermal Magnesium Therapy
Current H. pylori Protocol and December 13, 2016 Update (Pylopass) – BREAKING NEWS – Help Eliminate H. pylori with Pylopass
My current protocol consists of either Prevpac or Pylera (see September 2016 update below) and a personalized combination of biofilm-busting and natural antimicrobial agents. Below are the products I’m currently pulling from to create an individualized protocol. Having the correct biofilm and H. pylori protocol is just as important as having the correct dosage of the products used. To the degree that the balance of the three is in is to the degree that the entire protocol will succeed. This is why it’s always best to have a qualified coach and never self-treat.
- H. pylori Fight and PyloGuard (previously known as Pylopass)
- Natural Antimicrobial(s)
- S. boulardii
- Bladderwrack and/or Cranberry Extract w/D-Mannose
- Black Seed Oil
- A personalized Biofilm Protocol
Additional Data – Dissolve biofilms with fibrinolytic enzymes nattokinase and lumbrokinase By Dr. Peta Cohen. Effect of xylitol on an in vitro model of oral biofilm (PubMed). I have seen increased effectiveness since adding this to the protocol).
September 26, 2016 Update – IMPORTANT
PLEASE READ: Updated, Update – September 26, 2016, rev. August 19, 2014, original August 08, 2013
Since 2007, I have been helping patients with ulcers, gastritis, SIBO, and general GI distress caused by H. pylori and other biofilm-producing bacteria. In the beginning, getting rid of H. pylori was fairly easy, in my opinion. As time progressed, I noticed that the H. pylori-biofilm protocol, which up till now was very effective, was now becoming less and less effective, even with those receiving the H. pylori-biofilm protocol for the first time. There are now H. pylori strains that are ‘multiple drug-resistant’ even in those who have never been treated – Why? The linked article states that these mutations happen by chance. Meaning, medically, there is no real explanation for it, so they call it, “by chance.” Energetically, there is a very good explanation for it, at least for me anyway, based on the research done by Rupert Sheldrake, Ph.D., “Morphic Fields and Morphic Resonance.” Please read about his theory for further clarification.
The shift in effectiveness has compelled me to incorporate more than one round of products or add more products to the protocol. The end result has always been eradication, but it’s now taking more (products and/or time) to achieve this result. Also, there are many people reaching out to me and reporting that they have undergone triple and quadruple therapies to no avail. This suggests, in my mind, that the biofilm and the bacteria that form it are learning to defend themselves more effectively. They are adapting and mutating, genetically and energetically, to survive. Good for them and bad for us.
I theorize that with the introduction of hundreds of blogs, chat rooms, and websites devoted to H. pylori, H. pylori treatment, and biofilm, more and more people are self-treating and getting poor treatment outcomes. This self-treating is not killing the H. pylori or breaking up / busting through the biofilm but, to the contrary, making them both stronger by building up biofilm defense. Every time a bacterial biofilm is unsuccessfully eliminated it becomes more resistant to the next protocol. When this is combined with the theory of Morphic Fields, it’s no wonder why H. pylori and biofilm eradication is becoming harder and harder to achieve. The point of all of this is that there are still effective treatment options available; it may just take a little more time, and more products, allopathic (Prevpac or Pylera) and/or natural, may be required to get to the desired end result – H. pylori and biofilm eradication.
Personal note: don’t waste your money on Matula Herbal Formula. Many of the people who have contacted me have spent good money on the product with the hopes of it working. The Matula tea didn’t work, and they were unable to get the refund that was promised. Update: the company used to offer a 100% money-back guarantee if you were retested after using Matula tea and you still had H. pylori. They do not offer that guarantee anymore. I wonder why.
Lastly, I am not against the concept of self-treating per se. The issue is that the information available to you on biofilm and H. pylori eradication is not comprehensive or clear enough for you to be your own doctor or to successfully self-treat. I have always advocated and promoted that if you want to get better with or at something, you need a coach who is an expert in that field or subject. There are times and places where self-help is the best option, but biofilm and H. pylori treatment are not among them. This is just my opinion.
Biofilm Protocol Avoid or Add?
Avoid supplemental IRON during the biofilm protocol or when treating any bacterial infection. The abnormal presence of freely available iron will increase the rate of bacterial multiplication and tip the balance
in favor of the invading pathogen.
Avoid supplemental ZINC during the biofilm protocol, especially if you are taking antibiotics. Zinc is important in the breakdown of life-saving antibiotics. It is widely recognized that modern medicine is on the precipice of a microbial-induced disaster. The rise of bacterial strains (and enzymes) that are resistant to (and can inactivate) commonly used and recently developed antibiotics is risking nearly 80 years of progress in successfully treating once life-threatening bacterial infections. Much of this resistance is driven by a large class of enzymes localized to the bacterial surface termed Metallo-β-lactamases. These enzymes cleave the β-lactam ring of antibiotics that include penicillins, carbapenems, cephalosporins, and monobactams.
Avoid foods or supplemental – alginate, alginic acid, 1,3/1,6 beta-glucan, fructans, inulin, fructooligosaccharides, oligosaccharides, excess cellulose, and excess amylose. Strategy to combat biofilms: a focus on biofilm dispersal enzymes
Add? Data on Magnesium and Iron deficiency as a contributing factor to bacterial virulence and biofilm formation.
Magnesium – Studying the bacteria P. aeruginosa, a biofilm producer, researchers discovered a protein, the magnesium transporter E (MgtE). MgtE activity in P. aeruginosa appears to be influenced by the bacterium’s chemical surroundings, including the abnormal mucus in Cystic Fibrosis (CF) patients. Specifically, MgtE responds to fluctuations in magnesium levels. MgtE protein activates (increase in MgtE gene transcription) in the presence of LOW levels of magnesium, increasing biofilm formation, cytotoxicity, and bacterial virulence. This study was only conducted in a laboratory setting but may translate to low human tissue levels of magnesium as well.
in an email exchange with Dr. Gregory Anderson, one of the lead researchers for the above data.
Dr. Ettinger – “I’m assuming that the signaling is in response to “low” tissue magnesium levels. Is this correct?”
Dr. Anderson – “Yes, MgtE responds to low magnesium levels. Specifically, Mg limitation leads to an increase in mgtE gene transcription.”
Dr. Ettinger – “Did this mechanism reverse or stop when magnesium was increased, or did biofilm continue to form?”
Dr. Anderson – “We are now testing the effects of magnesium on biofilm formation. Don’t have conclusive results yet.”
Dr. Ettinger – “Have you studied any other minerals that may have a similar effect, like zinc or iron?”
Dr. Anderson – “We haven’t looked at zinc, iron, or other metals. MgtE only seems to be magnesium-responsive, though. It is known that iron limitation inhibits P. aeruginosa biofilm formation, as iron is important for biofilms.”
Magnesium is one of the most important minerals/chemicals in the body, and magnesium deficiency is one of the most common nutrient deficiencies in the world. Almost every RBC magnesium test I perform is at the lowest end of normal or, more commonly, below normal. Low levels of magnesium can lead to cardiovascular disease, weakened immunity, nerve and muscle conditions, migraine headaches, diabetes, chronic fatigue, fibromyalgia, and a myriad of other health maladies. So, it makes sense that in the presence of low magnesium levels, a biofilm-forming bacteria would become cocky and more aggressive. “when the cats are away, the mice will play.” Magnesium repletion therapy is one of the most common protocol recommendations I give to my patients. The magnesium bath below is the best way to rebuild tissue magnesium levels.
Magnesium Bath for Pain, Skin, Cancer, Autoimmune Disease, and More.
5 Little-Known Facts About Magnesium.
Iron – In addition, many bacterial pathogens have used the low concentration of iron present in the host as an important signal to enhance the expression of a wide variety of bacterial toxins and other virulence determinants. It’s a double-edged sword. On the one hand, iron may increase the growth rate of pathogenic bacteria, and on the other hand, low levels of iron may contribute to bacterial virulence and biofilm formation (as a protective mechanism). I guess the moral of the story is to stay healthy and not get put into a situation where a pathogenic bacteria can take up residence. Easier said than done, right? This thing may help to some degree – Epigenetics – Why Am I Sick, Why Am I Healthy.
Probiotics
Take a broad-spectrum, patented strain, probiotic and abundant amounts of prebiotics (polyphenols like clove, cinnamon, turmeric, raw cacao, and ginger; IAG [larch arabinogalactans]; flaxseed meal; onion, garlic, and fibrous fruits and vegetables. Probiotics, prebiotics, and high FODMAP foods may not be suitable for those with SIBO. This will need to be determined on a case-by-case basis. I like Xymogen’s ProbioMax 350DF, Xymogen ProbioMax Daily, and Progurt. VSL-3 can also be used (for a short period only) as well as Elaine Gotschall’s SCD™ yoghurt. These products will help crowd out the bad bacteria and disrupt and replace biofilm colonies along the mucous membrane.
Beneficial Yeast
Saccharomyces boulardii (S. boulardii) is another addition that will have positive benefits in any H. pylori, SIBO, or Candida eradication protocol.
A recent meta-analysis involving 14 RCTs (1671 patients) evaluated the role of probiotics in H. pylori eradication [Tong et al. 2007]. In patients with H. pylori infection, probiotic supplementation improved eradication rates and reduced treatment-related side effects and individual symptoms [Tong et al. 2007]. In this meta-analysis, only one RCT evaluated S. boulardii and found that it decreased the risk of diarrhea when given concomitantly to patients receiving triple eradication therapy for H. pylori [Duman et al. 2005]. S. boulardii induces morphologic changes in H. pylori cells consistent with cellular damage [Vandenplas et al. 2009] and was shown to cause a reduction in H. pylori colonization in infected children by 12% [Gotteland et al. 2005]. Of four RCTs testing S. boulardii in H. pylori infections, two were in children [Gotteland et al. 2005; Hurduc et al. 2009] and two in adults [Cindoruk et al. 2007; Cremonini et al. 2002]. Although there was no significant difference in H. pylori eradication between the S. boulardii and placebo groups, a significantly lower relative rate of AAD (16.1–25%) was observed. In a recent meta-analysis, the H. pylori eradication rate in the triple therapy group was 71% and increased significantly to 80% with S. boulardii supplementation [Szajewska et al. 2010]. Thus, S. boulardii may not be effective in eradicating H. pylori itself, but it is effective in reducing the side effects of the standard triple therapy (Prevpac).
Specific Additions Based on Condition (Not a complete list)
Candida albicans – SF722* (10-Undecenoic Acid) Thorne Research. This is as close as you can get to a medication and still be a natural substance. There are a few chat rooms blasting this product, based on who knows what – can’t make everyone happy. I’ve used SF722 for over 15 years, and it is amazing – never a problem! *Do not take SF722 if you are allergic to fish. Paracan MYC by Ecological Formulas, FC-Cidal, Dysbiocide, and ADP by Biotics Research are also dynamite products. There are many other amazing products that can be added to complement the SF722 and ADP. It’s really a matter of how many pills someone wants/doesn’t want to take per day or the severity of one’s condition, which will determine if or which additional products will be added. If the Candida albicans overgrowth is severe, has not responded to holistic methods, or has mutated into its more virulent hyphal form/fungal infection (nails, underarms, groin, or skin), Diflucan (fluconazole), a prescription medication, is my personal preference, but Nizoral (ketoconazole) can also be used. In Azole-resistant Candida albicans, lactoferrin must be added to either medication in order to increase its effectiveness. There are specific B-complex vitamins, minerals, and amino acids that possess synergistic properties, and I find them indispensable when taking Diflucan (fluconazole), Nizoral (ketoconazole), and for supporting candida (yeast/fungal) treatment, and die-off symptoms.
Chlamydia pneumonia, Klebsiella pneumonia, or Pseudomonas aeruginosa – One product I like is Pneumotrophin PMG by Standard Process, Inc. I use this because it helps direct the body’s attention to the affected area and assists the body’s healing efforts to the lung, where it’s needed most. Apex Energetics’ H-PLR and serrapeptase/nattokinase and/or bromelain are also mandatory additions. I also like to use OOrganik-15™ and Pneuma-Zyme™ by Biotics Research or Pneumatrophin PMG by Standard Process, as well as beta-glucan, colostrum, quercetin, and EGCG with some of my patients who manifest asthma, a chronic cough, emphysema-like symptoms, and mast cell (histamine) issues. Ig 26 by Xymogen, Restore, shilijit, and Progurt may also be utilized.
H. pylori Protocol – Heartburn/gastritis/GERD or achlorhydria or H. pylori?
Chronic bacterial prostatitis – EGCG and Quercitin and Bromelain combination by Now Foods decreases inflammation and oxidative stress in the prostate while increasing local concentrations of beta-endorphins. Apex Energetics’ H-PLR and iodine is also a mandatory addition. Iodoral, zinc, and vitamin D are also utilized.
Diet
Specific dietary restrictions or additions will most likely be implemented. These will be determined on a case-by-case basis based on many factors. After the desired result is achieved, a rebuilding and regeneration protocol will need to be implemented. This is as important as eliminating the biofilm.
Testing
Biofilm testing is available through Fry Laboratories. Fry Laboratories, L.L.C. is an independent clinical diagnostic and research laboratory located in Scottsdale, Arizona. We are committed to understanding chronic diseases and contributing to their cure through advancements in diagnostics and basic science research with an emphasis on chronic inflammatory diseases, vector-borne diseases, and their intersection. Our clinical diagnostic laboratory offers general and targeted immunology services in conjunction with standard and cutting-edge infectious disease detection and identification technologies. Our signature services include microscopy for visual identification and quantification of a wide range of blood-borne pathogens, co-infection serology, biofilm detection, and genus-wide molecular detection technology with sequencing for individualized species and/or strain identification. We participate in both CAP and API quality control programs and provide worldwide testing services.
Diseases of Interest: Chronic Fatigue Syndrome, Fibromyalgia, Gulf War Veterans Illness, Chronic Lyme Disease, ALS (Lou Gehrig’s Disease), Parkinson’s Disease, Multiple Sclerosis, Autism, Lupus, Ulcerative Colitis, Scleroderma, Rheumatoid Arthritis, Osteoarthritis, Crohn’s Disease.
Infections of Interest: Borrelia (Lyme), Babesia, Bartonella, Anaplasma, Ehrlichia, Q-fever (Coxiella), Toxoplasma, Rickettsia, Plasmodium, XMRV
Biofilm Protocol Tips for Bacterial and Yeast Infections
- Read This First – Epigenetics – Why Am I Sick, Why Am I Healthy
- Do not self-treat! I know this is hard, but it is the most important step. I’ve treated well over 300 H. pylori patients and even more biofilm-related conditions affecting the mouth, sinus, prostate, intestines, lungs, and skin. Muscle Response Testing and Applied Kinesiology has been a game changer when it comes to determining the correct combination of nutraceuticals to support each patient’s biofilm protocol. If it is at all possible, find a doctor who practices MRT or AK. Bring your biofilm-busting products to him or her for testing. Return any supplements you do not test for. In MRT or AK, your body will only test for the products that it energetically resonates with or is congruent with. These are the most effective products. Specifically tested by you, for you.
- Begin the protocol at least one month prior to adding in natural antimicrobials or antibiotics. This is very important when trying to eradicate chronic fungal and bacterial infections of the sinus cavity and prostate, as well as H. pylori infection of the stomach.
- Stay on the protocol during active treatment for bacterial, yeast, or fungal infections.
- Do not continue to take nutritional supplements that may contribute to biofilm formation or its structural integrity. These include but are not limited to Hydrochloric acid (HCl) in the case of H. pylori, calcium, and especially iron…
- Cover all of your bases. There does not exist, at all, a singular biofilm-busting product, period. Weakening or disrupting of bacterial or yeast biofilm through natural means, is in no way guaranteed. Regardless of how many biofilm-busting products are used. This is why I utilize a comprehensive top-to-bottom approach. Again, this is based both upon my education and Muscle Response Testing of all available products. By affecting each: quorum sensing and signaling molecules within the biofilm, and extracellular polymeric substance (ESP) matrix formation in the biofilm, the bacteria or yeast biofilm should be weakened. Research bacterial population density and biofilm.
- At a minimum, if you must self-treat, NAC, Lauracidin, colloidal silver, fucoidan, Interfase Plus (under a doctor’s supervision), and lactoferrin should be used. Additional enzymes such as serrapeptase and/or nattokinase, garlic, and ginseng will further strengthen the biofilm protocol. And if you want to go all-in, add some xylitol (not if you have IBS), BBF#1, and/or BBF#2 essential oils, as well as cranberry extract with d-mannose.
- Effect of Marine Polyunsaturated Fatty Acids on Biofilm Formation of Candida albicans and Candida dubliniensis
The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated. It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (EPA – 20:5 n-3), docosapentaenoic acid (DPA – 22:5 n-3) and docosahexaenoic acid (DHA – 22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both Candia albicans and Candida dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans. Ultrastructural differences, which may be due to increased oxidative stress, were observed between treated and untreated cells of C. albicans and C. dubliniensis with the formation of rough cell walls by both species and fibrillar structures in C. dubliniensis. These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.
Final Words
(1) Just because you may have tested positive for H. pylori does not mean all or any of your symptoms are caused by it.
(2.) Just because you tested negative after your protocol does not mean your symptoms will be gone or should be gone. See #1.
(3) Self-treating is usually a futile effort and one that just wastes a lot of time and money.
(4) If you’re not testing the best you are doing is guessing. If you are undergoing a protocol for any condition, based solely on symptoms and even worse, self-treating then don’t be surprised if it didn’t work.
(5) Don’t reinvent the wheel. If you need help in any area of your life in which you are not an expert, hire a coach.
Important: This post is not a substitute for medical advice or treatment and is for informational purposes only. Please consult with a physician before starting any nutritional or biofilm protocol on your own.
Research
Effect of ciprofloxacin and N-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces (PubMed)
Hello Dr. Ettinger,
Is it imperative that the biofilm supplements one is using to fight recalcitrant pathogens NOT be titrated up from a small starting dose, so that the bad bugs do not have time to defend themselves adequately against biofilm disruption? Even if I’m extremely sensitive, is the idea that I should be trying to start any biofilm disruptors at full doses from the beginning of treatment?
Thank you very much,
Carlo
Carlo,
There is far more to the reducing biofilm than merely using binders or disrupters. Biofilm is part of our bacterial make up. Pathogenic biofilm is a response not the cause.
Dr. Ettinger
Hello Dr Marcus
Apolactoferrin also destroys Biofilm??
I bought 3 bottles of Lactoferrin from Jarrow Formulas but it turned out to be Apolactoferrin
Can Lactoferrin + Interphase + Nattokinase be taken together at the same time or should I take the Lactoferrin Enzymes separately?
Thanks
Jorge,
Apolactoferrin is just a synthetic form of lactoferrin; they are interchangeable.
I can’t give you a specific recommendation, but I can say the 3 products mentioned do not react negatively if they all are taken together. Again, this is not a recommendation.
Respectfully,
Dr. Ettinger
Hi Dr. Marcus.
I’m taking huge amount of cellulase like 3g per day, 5g of amylase, 600mg of protease and lipase, some amount of serrapeptase and nattokinase. I’m taking NAC on an empty stomach also.
Do you think it’s enough? I don’t have hemicellulase, beta-glucosidase, chitonase unfortunately.
Thanks
Alexandre,
I’m sorry but I can’t give medical advice to non-patients.
Respectfully,
Dr. Etinger
Hello Dr. Marcus Ettinger
To this day you still think that it should not be supplemented with Zinc???
After seeing an article on your blog and check with my doctor I bought a Supplement with Pylopass but it contains Zinc L-Carnosine, do you think I shouldn’t take it (I have H.Pylori, Sibo and Other Negative Bacteria)????
And in case you need to take an Extra Zinc (Andy Cutler Protocol) what would you advise???
Thanks
Jorge,
Thank you for the question.
Zinc carnosine is a unique compound and I’m okay with that. An H. pylori protocol should not take that long and going off of a straight zinc supplement for a few days is not going to have a negative impact on one’s health.
Respectfully,
Dr. Ettinger
Hello dear Dr. Marcus Ettinger,
I tried to post before but I cannot find my post regarding H.pylori eradication. I have found a superior way to cure most gastro-intestinal infections and H.pylori in particular by prolonged water fasting and taking only a fiber psyllium husk. As simple and as ridiculous this might sound, this protocol I developed it after tones of trials, reading pudmed, digesting books from the east and the west and trying every supplement and probiotic even from Russia and Ukraine and Japan for H.pylori, including the rare LG21 probiotic from Japan. I found an interesting way to eradicate it after finding a thesis from Russia about using IL2 injection ( Interleukin 2 injection) to eradicate H.pylori and they compared it to triple therapy. IL2 gave a superior eradication rate 100% compared to the antibiotics. My idea was to find ways to increase IL2 naturally if possible. The only things I found was that fasting ( prolonged 1 week to 2 week) increases considerably IL2. Psyllium husks have an immunomodulatory effect as a fiber and also increase IL2. By doing the two, you can eradicate H.pylori and most bugs. Sometimes, the most effective remedies are the Simplest.
What you think? Also you can add Lion mane mushroom to increase Il2 and interferon gamma. Dr. Bruno Donatini claims he had also complete eradication of H.pylori with lion mane mushroom only.
Yahia,
Thank you for sharing your thoughts and personal experience.
Respectfully,
Dr. Ettinger
Hello..ive read good reviews about h-plr from apex..is that still recommended
Ed,
I use it sometimes, still. I rarely use it alone and only for specific situations.
Dr. Ettinger
Hello dear Dr. Marcus Ettinger,
what are your thoughts on Jim Humbles mms (chlorine dioxide) and mms2 (calcium hypochlorite) treatment protocols for different pathogens?
Do you think it would be effective for eradicating h pylori and biofilm, since resistance can not be developed to this oxidizers?
Have tried different combos for h pylori (metronidazole, clarithromycin, peptobismol,amoxcillin, nac, hydrogen peroxide iv and oral with dmso, probiotics). But still have pylori.
Currently thinking to try mms, also oregano oil with silver nanoparticles.
Thanks for attention!
Jessie,
This is just my opinion. I’m not a fan of MMS. It may help you, It may hurt you.
Kikuchi-Fujimoto disease presenting after consumption of ‘Miracle Mineral Solution’ (sodium chlorite)
Yes this comes from the FDA, so I take it with a grain of salt, but it is worth noting the potential of negative effects from MMS – FDA warns consumers about the dangerous and potentially life-threatening side effects of Miracle Mineral Solution
Respectfully,
Dr. Ettinger
Hello Good Doctor☺️
Do you take any sort of medical insurance?
Jennifer,
I’m sorry but I don’t.
I have been affected by a painless Bartholin Cyst for years now – which develops due to an obstruction in the duct that from which fluids release (from the Bartholin gland located at the opening of the vagina). Doctors are completely stumped and usually advise surgery or to leave it alone which causes further physical and emotional harm. During my research in discussion boards for treating this, many women have found success with serrapeptase and many also recommend garlic and silica which has worked for some, but many are still battling this issue for years and some have given up and had the entire gland removed or gotten surgery to create an alternate opening (which many times ends up closing back up or causing more issues with sensitivity). Somehow all of this eventually led me to biofilms. Due to the location of the blocked duct, and the remedies that have worked for some, I am beginning to think that this blocked may be due to biofilm formation within the channel… esp for blockages without the presence of infection. I am deeply committed to researching this as doctors have mainly given up and are telling women to just deal with it or keep having surgery in that area with a large chance that the issue will continue. I am thinking this protocol would be useful but am not sure if all of the steps would be required or how it might be adapted to the bartholin cyst. I would sincerely appreciate it if you could let me know what you think.
Jess,
Biofilm can be a component, especially if gram-negative bacteria or candida are involved. As far as advice, I can’t give you any. You provided your issue, but I know nothing about the rest of you, your labs, diet, sleep, co-founding factors… I’m sure I can help, but I would need a lot more data to be able to do so or tell you if I can’t help.
If you are interested, you can call (or email if out of the country) for a complimentary 15 minute consultation to see if we are a good fit. We can use Zoom if out of the country. 714-639-4360
Respectfully,
Dr. Ettinger
Hi Dr. Ettinger,
I had the H. pylori infection 7 months ago. I was retested and my breath test was negative but I was still have abdominal pain (mostly upper abdominal pain). I then found out I have candida overgrowth. I read that xylitol sugar works well for biofilms. Is it okay to be using xylitol as a sugar substitute when I’m baking? I’m avoiding sugar, gluten and dairy. I do eat gluten free grains.
Thank you.
Nadia,
Without a crystal ball, it would be impossible for me to give you an answer. Here is why: if you have SIBO as your symptom generator then high FODMAP’s can make things worse. ‘P’ stands for polyols which are sugar alcohols. They end in ‘ol’ Xylitol is a sugar alcohol. If you don’t have SIBO then it would be okay. That said, I have had cases of SIBO that were caused by too many polyols in the diet. The moral of the story is to find out what is causing your symptoms. If you need help, my practice is accepting new patients, in-office, and remote.
Respectfully,
Dr. Ettinger
Hello, Doctor, may I respectfully ask why you emphatically say “don’t self treat”? Great articles by the way. Thanks a bunch
Ian,
Unless you are an expert in the fields needed to successfully and skillfully treat your health problem you’re more likely to spend a ton of money and time on protocols that yield symptomatic relief at best, lacking any durable resolution. Yes, there are those who do obtain a durable resolution, but in 31 years of doing this, it’s not the norm.
We are as good as the coaches we surround ourselves with.
Respectfully,
Dr. Ettinger
I think you make a great point for more advanced cases in particular. It seems the more sick somebody is, the more likely a small change in their supplement, diet or lifestyle routine can potentially make them much worse. It’s not uncommon where I’ve heard on forums of people saying they started supplementing this or that or ate a certain food and it “screwed them up for a week, month,etc”. Some of the less chronic cases though could take herbals, certain strains of probiotics, or simply just clean up their diet and lifestyle and feel better for good
Ian,
“…simply just clean up their diet and lifestyle and feel better for good.”
Yes, you are correct. As simple as that sounds, most people can’t do it alone. 80-90% of my new patients tried various diets and lifestyle modifications, only to drop them out after they got the initial result they were looking for. They did not keep up the good habits that keep us in a more healthy state.
Having a good coach keeps one on the right path until good habits become second nature. This could be a doctor, pastor, financial advisor…
Take care,
Dr. Ettinger
Hello Sir, my name is Isaiah I have been troubled with miserable stomach problems for 4 years now and it all happened all of a sudden when I woke up one morning from not getting enough sleep the night before where I ate a slice of coconut cream pie and a granola bar(low sugar) before going to bed. When I woke up I took some barley grass powder mixed with ginger powder in water. I tried to start my day but my stomach was bloated so I waited and waited, nothing changed. From that point on my stomach has been bloated for 4 years with a feeling of fullness, sluggish slower metabolism/bowel moments, I still go but every time it feels like I haven’t fully evacuated my stomach and I am still bloated afterward, sometimes even more. I was told I have SIBO by western medicine doctors (breathe test) and spleen deficiency by a Chinese acupuncturist (didn’t receive any acupuncture) just told her my symptoms. I also have a small black rash on the side on my stomach that itches me every day. I’m not sure if its SIBO, candida, or something else. I also did a stool test and they ruled out parasites. However, through taking many antifungals over the years one time I saw things on my stool that google labels candida (it looked like little cotton balls with red legs) I’m just lost and depressed I need help do you have any suggestions, please help.
Isaiah,
I can help. Please call me at my office and we can discuss your case and how to become a distance client. 714 – 639 – 4360
Respectfully,
Dr. Ettinger
lots of great info,
i just wish you had written it a bit more coherently.
all the ‘updated information’ and extra bits here and there, it becomes quite complicated.
I’m pretty sure your info could be better written.
I’m sure this info could be more accessible.
I truly do appreciate the vast amount of time you have spent doing this, but I only wish you had presented it more cleanly.
Kind Regards,
James
James,
I agree. I wrote my biofilm and H. pylori protocol posts 11 years ago and WordPress/blogs were not what they are today. My newer blogs are shorter and more to the point. Thank you for the comment.
Dr. Ettinger
Hello, I have been suffering from a rheumatic arthritis type pain for almost 12, since age 39. Just before that I was treated for H. Pylori. Over the past 12 years all my tests come back normal for any inflammation. I do have HLA B 27 positive though. Could my arthritis be reactive? Although another h pylori test by biopsy was negative a year ago, could the h pylori be protected by biofilm?
Jen,
If you had an H. pylori breath test or stool antigen test and it came back negative then it’s 99.9% negative. Reactive arthritis, if that’s what’s going on can be activated by MANY different epigenetic factors. If you need a detective to help you solve your case, I can help. Please feel free to reach out to me at 714-639-4360
Respectfully,
Dr. Ettinger
Hi Dr. Ettinger,
Can SF722 and Interphase Plus be taken at the same time of day (i.e. swallowed at the same time) or should one wait a certain amount of minutes/hours between them?
Many thanks!
In my opinion, InterPhase should always be taken alone and on an empty stomach.
Thank you Dr. Ettinger! How long do you recommend waiting between Interphase and SF722? 30 min? 1 hour?
An hour.
Hi Dr. Ettinger!
Have been on a very clean diet since 1,5 year (occasionally cheating) ie gluten and dairy free, fresh food with lots of veggies and fruits plus good meat. Using lots of supplements like nac, ala, dandelion, vitamin and minerals like C, D, A, K, B, Mg, Zn, Co, etc under my holistic MD’s recommendations. Doing saunas and smoothies too for detoxing.
All the hormon tests, blood labs are normal. I am female btw. But having extreme scalp tendency together with hair loss and graying hair (even though I use different organic sulfat free shampoos or different organic hair dye), never ending acne on my back and all over the face (not cystic but bad) and itching anus.
Recently have been considering to include smooth coffee enemas and/or black walnut/wormwood supplements.
I really need your help, since I did almost everything without seeing those issues gone… Would you recommend to do a smoot coffee enamas and/or wormwood protocols? Or any other thing that is affordable and effective? Would be so much appreciated!
Many thanks!
Melissa,
From your lack of healing and your specific list of symptoms, I’ll guess that you spend VERY little time, lightly clothed, in the sun. Here is free advice and I hope it helps. Below are four health datums I live by and use to help my patients. All of this can be found on the home page of my website.
1. Biology DOES NOT give a shit about your philosophy when you’re sick.
2. And, you will NEVER get better in the same environment that made you sick.
3. And, the Sun IS nature’s original and the most powerful vaccine against disease.
4. And, your environment actually controls your physiology to a greater degree than anything you will ever put into your mouth (medications, supplements or food)…
The truth is LIGHT, WATER, and MAGNETISM have been here since time immemorial. These three forces have literally shaped human existence and all that surrounds us. So logically, why would it be so hard to believe that the avoidance or alteration of these three forces could cause disease or that by harnessing them, health could be created or restored?
Do you see any difference in what you and your MD are doing compared to what I would be doing? Yes, I do use supplementation, but the 4 datums come first.
I hope this helps and if this makes sense and you would like my help, I’m here for you. I help people all over the world to get back to health, even those that say they’ve seen everyone and tried everything.
714 – 639 – 4360 or info @ advancedhealing .com
Respectfully,
Dr. Ettinger
Hi Dr Ettinger,
I have recently had an Organic Acids Test which showed high levels of multistrain clostridia, high yeast, High oxalate and high dopac. I have been searching for this answer now for 10 years. I can now only eat about 10-15 foods and remain functional. I have declined to the stage of some foods triggering borderline psychosis and suicidal tendancies. I have had great success working with a homeopath this week so this work is early days. Do you work with patients with these kind of test results and severe food reactions? I can not tolerate any plant based medicine except aloe vera and a very few others. I have had major collapses due to some foods and morphine treatment in hospital which i would consider near death. I am desperate to get help. As soon as in introduced a few teaspoons of probiotics, I had a severe reaction. I feel stuck in this situation. thanks you so much
Hannah,
I’ve helped many patients in similar situations as the one you’re in now. That said every person is different and will need a different protocol to get back to health. If you are lost for answers and would like a coach to help you, I’m here for you. Please feel free to reach out to me and I’ll groove you in on how to become a distance patient unless you are here in So. Cal. 714-639-4360
Respectfully,
Hi Doc – I have a question in re: SARS-Cov-2.
I have recently digested well over 2,000-3,000 pages related to the latest SARS-Cov-2 science / case studies / protocol efficacy, etc., and routinely discuss protocols (over last 20 years) with my integrative med providers.
My family is following a strong regimen of Liposomal C + “Pauling C” based on “1/2 life” to smooth out daily plasma level fluctuations. Also taking monolaurin to help eliminate any chronic inflammation (molds, Lymes, West Nile, other difficult to diagnose) in preparation of contracting COVID-19, and substantially up-dosng when symptomatic. Concurrently, we’d up-dose vitamin C form our 2,000-3,000 daily, well into the 10,000mg+ levels.
BUT – Isaacs et. al.* found that monolaurin disintegrates virus lipid layer to kill virus. Logic would then say that monolaurin would destroy the lipid encapsulation of liposomal C.
Do you know of any studies showing the effect of monolaurin’s mechanism (or no effect) on the lipid encapsulation layer of vitamin C?
I’m trying to find indications as to whether monolaurin would ameliorate vitamin C’s beneficial liposomal mechanism. This would be interesting in light of COVID prevention/treatment protocols.
Perhaps they should not be taken together? But, both are shown to work against COVID-19.
Thoughts?
*Thormar, H; Isaacs, C E; Brown, H R; Barshatzky, M R; Pessolano, T (1 January 1987). “Inactivation of enveloped viruses and killing of cells by fatty acids and monoglycerides”. Antimicrobial Agents and Chemotherapy.
Thanks in advance – be safe out there.
Andy,
Please read this paper Effects of Vitamin C Supplementation on Plasma and Urinary Vitamin C Concentration in Korean Women
I don’t recommend high dose vitamin C unless given by IV, for conditions like cancer and where high “oxidant” therapy is needed. To me, it’s better to take some throughout the day up to say 2,000mg’s. Vitamin C is recycled via GSH, so having plenty of NAC is more important, to me than having high vitamin C. Also, too much vitamin C (antioxidants) MAY? reduce mitochondria produced ROS, which is needed for mitochondria biogenesis. Cheap ascorbic acid is all I carry at the office. Mineral ascorbate is good too if the person is low in alkaline minerals. As a side note, I’ve never taken vitamin C in my daily supplement protocol. I have taken NAC for 26+ years.
Liposomal vitamin C utilizes lecithin. Lauric acid is not going to dissolve lecithin. You are good to go with liposomal vitamin C.
Respectfully,
Dr. Ettinger
Hi again!
I still have one question left that you can answer it, since it is not in a form of a medical advice.
Why is that this statement: “Avoid supplemental ZINC” is something that can be found only on your website, while everybody else are saying the complete opposite?
Is there a specific form of Zinc that you suggest, or you’re don’t suggest supplementing it at all, or it is something that we should avoid at a certain part of the protocol?
Thanks!
Vladimir,
Did you read the journal article linked to what you are referencing?
Avoid supplemental ZINC during the biofilm protocol, especially if you are taking antibiotics. Zinc is important in the break-down of life-saving antibiotics. It is widely recognized that modern medicine is on the precipice of a microbial-induced disaster. The rise of bacterial strains (and enzymes) that are resistant to (and can inactivate) commonly used and recently developed antibiotics is risking nearly 80 years of progress in successfully treating once life-threatening bacterial infections. Much of this resistance is driven by a large class of enzymes localized to the bacterial surface termed Metallo-β-lactamases. These enzymes cleave the β-lactam ring of antibiotics that include the penicillins, carbapenems, cephalosporins, and monobactams.
The flip side – are you completely zinc depleted? If so, then maybe thaat can contribute biofilm formation. Yo have a choice, take zinc and increase bacterial virulence and decrease treatment effectiveness or take zinc in hopes you suppress biofilm formation. Since I’m in the know; if you already have symptoms you already have biofilm and this conversation has a clear answer.
Helicobacter pylori Biofilm Formation Is Differentially Affected by Common Culture Conditions, and Proteins Play a Central Role in the Biofilm Matrix